产品货号：
GS2171
中文名称：
BstX2I内切酶
英文名称：
BstX2I
产品规格：
250U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：BstX2I内切酶，BstX2I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl. |
| Recognition sites | R↑GATCY |
| 来源 | Isolated from E.coli strain that carries the cloned BstX2I gene from Bacillus stearothermophilus X2 |
| Assayed on | Lambda DNA |
| Optimal buffer | G?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.) |
| Optimal temperature | 60℃ |
| Storage conditions | 10mMTris-HCl(pH 7.6);100 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 60℃. |
| Methlation effect | Not blocked by Dam-methylation?(GmATC):RGATCY |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BstYI,MflI,PsuI,XhoII |
相关搜索：BstX2I内切酶，BstX2I限制性内切酶